|Lab Managers:||Christa Lanz, Heike Budde|
|Phone:||+49 7071 601-348, +49 7071 601-1446|
The Genome Center Facility and the Department of Molecular Biology (Detlef Weigel) introduced next-generation DNA sequencing to our institute as early as 2007. By now, sequencing-based approaches are crucial for the large majority of our research groups. These include de novo genome assemblies, variant calling, metagenomics, chromatin analysis, full-length and short read transcriptomics, small RNA analysis and single-cell applications from organisms as diverse as vertebrates, insects, worms, plants, brown algae, fungi, ciliates and microbes.
The Genome Center hosts a Sequel II instrument from Pacific Biosciences for high-fidelity single-molecule real-time (SMRT) sequencing of DNA fragments with many thousand nucleotides in length. The facility also operates a HiSeq 3000 platform from Illumina routinely generating paired-end short-read data (2 x 150 nucleotides) from small DNA fragments in the order of 800 gigabases per run. Finally, an Illumina MiSeq sequencer generates variable read lengths for small-scale projects.
The Genome Center assures fast, reliable and high-quality sequencing services and supports the development of new application protocols. The facility trains internal and external scientists in preparing libraries, providing lab-space and assistance with technical equipment for library preparation and quality control.